Cellular heterogeneity is a defining feature of biological systems, with implications in disease processes and therapeutic response variability. Many disease models also comprise an assortment of cell types that all contribute to disease phenotype. While advances in sequencing have improved our ability to characterize distinct cell types at the single-cell level, few tools can non-invasively monitor real-time heterogeneity in large cell populations without the need for dyes or labels.

In this application note, you will explore:

  • How CytoTronics’ electrical imaging platform can create high-dimensional phenotypic fingerprints for various cell types
  • Experimental results demonstrating the platform’s ability to distinguish epithelial-like and mesenchymal cell types in a model of epithelial-to-mesenchymal transition (EMT)
  • How longitudinal population distribution data can be used to infer contributions of each cell type to a mixed culture at different time points

Comparing epithelial-like cells with cells that have undergone EMT.