Knowing exactly how a compound affects the body is critical to assessing its efficacy and safety; however, most cytotoxicity assays offer only end-point snapshots of cell death. Determining a compound’s mechanism of action (MoA) requires a multi-modal view of cell phenotype over time, measuring changes in behavior, morphology, and function.

In this case study, you will explore:

  • The distinct phenotypic fingerprints of cells responding to 8 cytotoxic compounds targeting different pathways
  • The benefit of label-free, real-time impedance measurements for revealing subtle functional and morphological changes beyond cell death alone
  • How CytoTronics’ electrical imaging platform enables determination of compound response kinetics and mechanism of action

Effect of different compounds on cell culture confluence over time.