In vitro modeling of vascular biology using human umbilical vein endothelial cells

Endothelial cell barriers help regulate the exchange of biomaterials between the bloodstream and surrounding tissues, playing important roles in processes like angiogenesis, inflammation, and tissue repair. Studying tissue barrier function in vitro is critical to understanding pathological and developmental processes in vascular biology.

In this case study, you will explore:

• How CytoTronics’ electrical imaging platform enables real-time, multiparametric measurement of changes in culture health and barrier integrity
• The use of impedance measurements to quantify dose-dependent changes in tissue barrier after treatment with thrombin, a key mediator of vascular permeability
• How multimodal data provide mechanistic insight into vascular behavior
• The value of longitudinal, label-free assays for modeling both reversible and sustained changes in vascular biology