Longitudinal measurements of HUVEC cells upon addition of thrombin (0, 0.25, 0.5, 1, 2, 4, 8 U/mL). (A) Confluence, barrier, cell-cell adhesion, and attachment as measured for 8 hours after thrombin addition as indicated by the dashed line. Shaded regions indicate standard error of 3–6 technical replicates. (B) Electrical images for three conditions (0, 2, and 4 U/mL) prior to thrombin addition (0 hours) and post thrombin addition (+1 and +6 hours) where red represents barrier and green represents attachment.